The principal objective of this study was to evaluate the utility of on-line in-vivo chlorophyll determinations in conjunction with on-line pH and dissolved oxygen (DO) concentration measurements for detecting the presence of algae in natural waters. Specific objectives were to: determine the suitability of in-vivo fluorescence (IVF) for on-line monitoring of chlorophyll a; determine the utility of on-line chlorophyll a, pH, and dissolved oxygen (DO) measurements for detecting the presence of algae in natural waters; and, correlate chlorophyll a data with algal cell count and biovolume data. Source waters for the City of High Point, NC, drinking water treatment plant were monitored. Water from City Lake was withdrawn from a depth of 0.5 m at the intake structure near the dam. Water from Oak Hollow Lake, the back-up water source, was also withdrawn from a 0.5 m depth at the intake structure. City Lake was being aerated during the on-line experiments to maintain destratification and to control harmful algae blooms. Includes 11 references, tables, figures.