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Design, Evaluation, and Molecular Microbial Ecology of a Pilot-Scale Biologically Active Filter for Ammonia Oxidation 中试规模氨氧化生物活性过滤器的设计、评价和分子微生物生态学
发布日期: 2009-11-01
本powerpoint演示首先简要概述了氨和生物处理。研究目的是:描述全尺寸生物活性过滤器的特性 利用分子技术识别主要疾病 提出并确定责任人 硝化作用;在受控条件下设计和运行先导过滤器 评估作战人员作用的条件 功能条件;和 观察微生物种群的变化 对先导滤清器工作条件的响应。方法: ICP-MS和ICP-AES;SmartChem比色法;EM、XRD、EDS;还有,钳工。 分子方法:培养非依赖性16s rDNA基因(11F, 1492R),氨 单加氧酶基因 (AOB和AOA),需氧亚砷酸盐 氧化酶基因;以及培养依赖性HPC、大肠杆菌、肠球菌、需氧内孢子和亚砷酸盐氧化剂。生物活性过滤被认为是一种高效、可持续的过滤方法 高浓度水源水处理技术 氨: 反冲洗快速恢复; 对操作条件的变化不敏感; 氧化氨>90%,完全转化为 硝酸盐; 无致病微生物/指标; 降低运营成本; 亚硝基单胞菌属和亚硝基螺属是主要属 参与硝化作用;和 79%的16s多样性是由单身人士驱动的,44%的16s多样性是由单身人士驱动的 阿莫阿。包括数字。
This powerpoint presentation begins by providing a brief overview of ammonia and biological treatment. Study objectives were to: characterize a full-scale biologically active filter using molecular techniques to identify major genera present and determine those responsible for nitrification; design and operate pilot filters under controlled conditions to evaluate the role of operational conditions on functionality; and, observe microbial population changes in response to operating conditions in the pilot filters. Methods: ICP-MS and ICP-AES; SmartChem-Colorimetry; EM, XRD, EDS; and, benchwork. Molecular methods: culture-independent 16s rDNA gene (11F, 1492R), Ammonia monooxygenase gene (AOB and AOA), Aerobic arsenite oxidase gene; and, culture-dependent HPC, E. coli, enteroccoci, aerobic endospores, and arsenite oxidizers. Biological active filtration was found to be an efficient and sustainable technology for treatment of source waters with elevated ammonia: rapid recovery from backwash; insensitive to changes in operating conditions; oxidize ammonia >90% with complete conversion to nitrate; no pathogenic organisms/indicators; lower operating costs; nitrosomonas and Nitrospira are the major genera involved in nitrification; and, 79% of 16s diversity driven by singletons, 44% in amoA. Includes figures.
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发布单位或类别: 美国-美国给水工程协会
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