Microbiology of the food chain. Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli-Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide
食物链的微生物学 β-葡萄糖醛酸酶阳性大肠杆菌计数的水平方法 5-溴-4-氯-3-吲哚基-D-葡萄糖醛酸的检测和最大或然数技术
BS EN ISO 16649-3:2015 specifies a horizontal method for the detection and enumeration of?-glucuronidase
positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most
probable number (MPN) after incubation at (37 ± 1) °C, then at (44 ± 1) °C. This part of ISO 16649 is
applicable to the following:products intended for human consumption and the feeding of animals;environmental samples in the area of food production and food handling.The method is suitable for the enumeration of cells of E. coli that might have been subjected to stress
arising from dehydration, freezing, and exposure to a saline (such as marine) environment or damage
by disinfectants such as chlorine-containing products.A limitation of the applicability of this part of ISO 16649 is imposed by the susceptibility of the method
to a large degree of variability. The method is intended to be applied and the results interpreted in the
light of the information given in Clause 11.This method has not been fully evaluated for all matrices (e.g. for milk and milk products). ISO 7251 is
intended to be used for milk and milk products.Cross References:ISO 6887-1ISO 6887-2ISO 6887-3ISO 6887-4ISO 6887-5ISO 6887-6ISO 7218ISO 11133ISO 7251All current amendments available at time of purchase are included with the purchase of this document.