这项工作的目的是评估基于培养的定量聚合酶 基于链式反应（PCR）的检测水中耶尔森菌的方法 发生在加拿大安大略省西南部的格兰德河流域。这个分水岭是 作为50万人的饮用水源，以及娱乐用途。信息技术 也是加拿大受影响最严重的流域之一，并从这两个流域获得投入 农业和城市活动。年，沿着格兰德河选择了五个采样点 基奇纳和滑铁卢城市周围的地区。从每一处收集地表水 每隔一周对这些网站进行一次检查。 在这项研究中，以培养为基础的工作包括比较四种不同的浓缩肉汤 以及检查碱性处理在每个浓缩液中培养的培养物的效果。 这些方法是从文献中选择的，最初是为 通常用于从食物和临床样本中分离小肠结肠炎耶尔森菌。这些方法 还没有在水上进行广泛的测试。结果表明，其中一种浓缩液 经测试，改性胰蛋白酶大豆肉汤（mTSB）似乎更有可能回收Y。 小肠结肠炎比其他疾病更严重。此外，碱性处理被发现是有效的。 因此，mTSB浓缩液在经过或不经过碱性处理后 在头孢磺啶-伊尔加桑新生霉素（CIN）琼脂上生长，该琼脂是Y。 小肠结肠炎菌被用来从格兰德河的地表水样本中分离耶尔森菌。 通过一系列生化试验确认了推定的耶尔森菌分离株。证实 从这两个地区采集的154份地表水样本中，有39份（25%）分离到耶尔森菌 2006年4月和2007年5月。然后对分离的菌株进行亚型，以确定其种类和分布 生物型。所分离的菌株均为小肠结肠炎Y.菌。被隔离的物种包括 Y.aldovae、Y.bercovieri、Y.frederiksenii和Y.intermedia都被认为是非致病性的 种以前在水生动物中发现过非致病性耶尔森菌 环境。2006年分离的菌株也对这两种毒力基因进行了筛选 在基于分子的方法中有针对性，而地表水分离物没有 包含任何一种基因。包括9篇参考文献。

The objective of this work was to evaluate culture-based and quantitative polymerase chain reaction (PCR)-based methods for the detection of Yersinia spp. in water, by examining its occurrence in the Grand River watershed in Southwestern Ontario, Canada. This watershed is used as a drinking water source for up to 500,000 people, as well as for recreational purposes. It is also one of the most heavily impacted watersheds in Canada and receives inputs from both agricultural and urban activities. Five sampling locations were selected along the Grand River in the area surrounding the cities of Kitchener and Waterloo. Surface water was collected from each of these sites every other week. In this study, culture-based work involved comparing four different enrichment broths as well as examining the effect of an alkaline treatment of cultures grown in each enrichment broth. The methods were selected from the literature and were originally developed for and are commonly used for isolating Y. enterocolitica from food and clinical samples. These methods have not been tested extensively on water. Results showed that one of the enrichment broths tested, modified tryptic soy broth (mTSB), appeared to have more potential for recovery of Y. enterocolitica than the others. Additionally, an alkaline treatment was found to be effective. Consequently, the mTSB enrichment broth with and without an alkaline treatment followed by growth on Cefsulodin-Irgasan-Novobiocin (CIN) agar, a commonly used selective agar for Y. enterocolitica, was used to isolate Yersinia from surface water samples from the Grand River. Presumptive Yersinia isolates were confirmed with a series of biochemical tests. Confirmed Yersinia strains were isolated from 39 out of 154 (25%) surface water samples collected between April 2006 and May 2007. Isolated strains were then sub-typed to determine their species and biotype. None of the strains isolated were Y. enterocolitica. Species that were isolated included Y. aldovae, Y. bercovieri, Y. frederiksenii and Y. intermedia, all typically considered to be nonpathogenic species. Non-pathogenic Yersinia species have previously been found in aquatic environments. Strains isolated in 2006 were also screened for the two virulence genes targeted in the molecular-based methods and the surface water isolates did not contain either gene. Includes 9 references.