1.1 本试验方法包括定量测定液态烃燃料的生物碳含量，重点是使用液体闪烁计数（LSC）在典型炼油厂生产的燃料。该方法旨在产生与试验方法类似的结果 第6866页 方法C，用于低骤冷样品，无需苯合成。其目的是能够使用生产的数据向监管机构报告炼油厂产品的生物碳含量，并监控炼油厂的运行。该方法不涉及监管报告或燃料性能。 1.2 当生物进料与石油在一个反应器内共同处理时，需要该方法来支持炼油厂的操作，重点是100 % 生物碳或更少（不适用于 14 C标记的物种）。它允许炼油厂向环境保护局（EPA）或加州空气资源委员会（CARB）等监管机构报告炼油厂产品的生物碳含量，以遵守可再生燃料标准（RFS）或低碳燃料标准（LCFS）等监管法规。 1.3 本试验方法适用于含有1 % 至100 % 其中可以使用不含可测量碳的类似基质样品实验确定仪器背景- 14 1.4 尽管参考文献 ( 1- 11 ) . 2. 但是，对上述试验方法的样品制备进行了说明。此处不包括仪器操作的详细信息。最好从所用特定仪器的制造商处获得。 1.5 方法执行的先决条件要求- 本试验方法使用人造碳-14( 14 C） 在方法内。应特别注意防止实验室污染高架 14 C、 一旦进入实验室，人工 14 C会污染各种实验室表面，从而导致人为的高样本生物碳测量。 如果强力清洁试图移除人工 14 来自实验室的C不成功，仪器和样品制备可能必须转移到远离污染的新实验室，或者实验室可能必须依赖外部第三方实验室进行分析。该方法中包含了特定的程序步骤，可将样品和实验室污染的风险降至最低。擦拭试验和质量保证样品可验证无污染。如果实验室或仪器受到污染，应执行严格的清洁规程，在实验室和仪器没有污染之前，不得恢复分析。 接受的要求包括: 1.5.1 使用高架 14 C样品放置在远离仪器的单独和规定区域，并制备任何未加标的样品。 1.5.2 使用单独的人员准备加标样品和未加标样品。 1.5.3 使用单独的实验室空间和单独的HVAC系统处理加标和未加标样品。使用具有单独排气通风的单独通风柜满足此要求。 1.5.4 每周擦拭测试 14 C样品处理区，用于检测实验室污染。 1.6 本标准并不旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前建立适当的安全、健康和环境实践，并确定监管限制的适用性。 1.7 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 =====意义和用途====== 5.1 该试验方法为碳源与CO直接平衡的材料提供了准确的生物基/生物源碳含量结果 2. 在呼吸或新陈代谢停止时，如在田里收获作物或草时。需要特别考虑将测试方法应用于源自非人工环境的材料- 自然水平 14 或者如果生物饲料是在几年的过程中生长的，例如树木，并且含有“炸弹碳” 2. 人工环境中的吸收超出了本标准的当前范围。 5.2 该方法使用LSC技术，使用未经改性的样品碳来量化液态烃燃料的生物基含量。它被设计为能够并入炼油厂实验室，以支持生物进料和石油共加工或混合操作，从而确定中间产品或成品的生物碳含量。测试结果可用于优化内部参数或向监管机构报告。 5.3 该方法的使用要求可以生成纯石油基样品，该样品具有与要分析的每种产品或物流相似的基质。例如，汽油和柴油具有非常不同的基质，可能需要对每种基质使用不同的背景测量。提到 10.2 关于如何确定相同的背景样品是否可以用于多于一个产品/流。

1.1 This test method covers quantitatively determining biocarbon content of liquid hydrocarbon fuels with a focus on those produced in a typical petroleum refinery using liquid scintillation counting (LSC). The method is designed to generate analogous results as Test Method D6866 Method C, for low quench samples, without the need of benzene synthesis. The purpose is to be able to use the produced data to report biocarbon content of refinery products to regulatory agencies and monitor refinery operation. The method does not address regulatory reporting or fuel performance. 1.2 The method is needed to support refinery operations when bio-feeds are co-processed with petroleum within a reactor with a focus on samples with 100 % biocarbon or less (not for 14 C labeled species). It allows refineries to report the biocarbon content of refinery products to regulatory agencies such as the Environmental Protection Agency (EPA) or California Air Resources Board (CARB) to comply with regulatory statutes such as The Renewable Fuel Standard (RFS) or Low Carbon Fuel Standard (LCFS). 1.3 This test method is applicable to any liquid fuel product, petroleum based (pure hydrocarbon), biobased (such as renewable diesel or those that can contain oxygenates such as ethanol), or blends, that contain 1 % to 100 % by mass biocarbon where an instrument background can be experimentally determined using a sample of similar matrix that contains no measurable carbon-14. 1.4 This test method makes no attempt to teach the basic principles of the instrumentation used although minimum requirements for instrument selection are referenced in Refs ( 1- 11 ) . 2 However, the preparation of samples for the above test methods is described. No details of instrument operation are included here. These are best obtained from the manufacturer of the specific instrument in use. 1.5 Pre-Requisite Requirements For Method Execution— This test method uses artificial carbon-14 ( 14 C) within the method. Great care shall be taken to prevent laboratory contamination of the elevated 14 C. Once in the laboratory, artificial 14 C can contaminate a variety of laboratory surfaces that can lead to artificially high sample biocarbon measurements. If vigorous cleaning attempts to remove the artificial 14 C from a laboratory are unsuccessful, instrumentation and sample preparation may have to be moved to a new laboratory away from the contamination or the laboratory may have to rely on outside third-party labs for analysis. Specific procedural steps have been incorporated into this method that minimize the risk of sample and lab contamination. Wipe tests and quality assurance samples can validate absence of contamination. In the event of contamination in the laboratory or instrument, vigorous cleaning protocols shall be implemented, and analysis cannot be resumed until the lab and instrument are free of contamination. Accepted requirements are: 1.5.1 Working with the elevated 14 C samples in a separate and defined area away from the instrument and the preparation of any non-spiked samples. 1.5.2 Using separate personnel to prepare the spiked samples and non-spiked samples. 1.5.3 Using separate laboratory spaces with separate HVAC systems for the handling of spiked and non-spiked samples. The use of separate fume hoods that have separate exhaust ventilation satisfies this requirement. 1.5.4 Weekly wipe tests of 14 C sample handling area(s) to detect lab contamination. 1.6 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.7 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This test method provides accurate biobased/biogenic carbon content results to materials whose carbon source was directly in equilibrium with CO 2 in the atmosphere at the time of cessation of respiration or metabolism, such as the harvesting of a crop or grass living in a field. Special considerations are needed to apply the testing method to materials originating from within artificial environments with non-natural levels of 14 C or if the biofeed was grown over the course of several years such as trees and contains “bomb-carbon.” Application of these test methods to materials derived from CO 2 uptake within artificial environments is beyond the present scope of this standard. 5.2 This method uses LSC techniques to quantify the biobased content of a liquid hydrocarbon fuels using sample carbon that has been unmodified. It is designed to be able to incorporate into a refinery laboratory to support biofeed and petroleum coprocessing or blending operations to determine the biocarbon content of the intermediate or finished products. The test results can then be used for optimizing internal parameters or reporting to regulatory agencies. 5.3 The use of this method requires that a pure petroleum-based sample can be generated that has a similar matrix to each product or stream to be analyzed. For example, gasoline and diesel have very different matrices and will likely require the use of different background measurements for each. Refer to 10.2 for how to determine if the same background sample can be used for more than one product/stream.