本文介绍了用梯度洗脱高效液相色谱法测定菜籽和菜籽粕中硫代葡萄糖苷含量的方法。本方法在菜籽和菜籽粕（油菜、甘蓝型油菜和芥菜）上进行了试验，但适用于其他植物材料，前提是本文件中描述了先前鉴定的硫代葡萄糖苷。相反，相关硫代葡萄糖苷（s）未进行定量分析。注:本方法不确定葡萄糖分子上取代的硫代葡萄糖苷，但这些化合物在商业菜籽和菜籽粕中的重要性很小。附录A给出了梯度洗脱高效液相色谱法实验室间试验的结果。附录B介绍了如何检查制备的内标溶液的滴定度。附录C介绍了如何制备和测试纯化的硫酸酯酶溶液以及如何在离子交换柱上检查脱硫步骤。附录D给出了高效液相色谱和色谱柱性能标准鉴定。油菜籽中硫代葡萄糖苷的含量也可以用等比例洗脱模式进行分析。这需要对方法（内部、标准、高效液相色谱柱和高效液相色谱缓冲液）进行一些修改，如附件E所述。

This document specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution. This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates previously identified are described in this document. On the contrary, the quantitative analysis of the concerned glucosinolate(s) is not carried out. NOTE This method does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal. Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method. Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents how to prepare and test the purified sulfatase solution and how to check the desulphation step on the ion exchange column. Annex D presents the HPLC and column performance criteria qualification. The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers), as described in Annex E.