美国的地下水可能受到各种来源的粪便污染。该分析的目的是探讨季节趋势，并检查PCR和不同温度组的培养方法之间是否存在任何关系。从35个州的448个地点（井）采集了地下水样本。在448个地点中，有25个进行了多次抽样。使用细胞培养试验检测每个样本的病毒感染性，使用RT-PCR检测病毒核酸的存在，使用三种不同细菌宿主的噬菌体，总大肠菌群、肠球菌、产气荚膜梭菌、总有机碳、各种矿物质和金属。包括11个参考文献、表格、图表。

Groundwater of the United States may be subjected to fecal contamination from avariety of sources. The objective of this analysis was to address seasonal trendsand to examine whether there was any relationship between PCR and culturablemethods in different temperature groups. Groundwater samples were collected from448 sites (wells) in 35 states. Of the 448 sites, 25 were sampled more thanonce. Each sample was assayed for virus infectivity using a cell culture assay,the presence of virus nucleic acid using RT-PCR, bacteriophage using threedifferent bacteria hosts, total coliforms, enterococci, Clostridium perfringens,total organic carbon, various minerals, and metals. Includes 11 references, table, figures.