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Standard Test Method for Quantification of Culturable Waterborne Bacteria Using a Defined Culture Medium Coated Plate 用规定的培养基包衣板定量测定可培养的水性细菌的标准试验方法
发布日期: 2023-07-01
1.1 该试验方法描述了一种简单的程序,用于定量饮用水(例如饮用水、瓶装水和牙科用水)和非饮用水(如冷却塔)中可培养的水性细菌。 1.1.1 EasyDisc 2. 3. 板格式设计为在47毫米网格板上测试1毫升水样,该网格板含有嵌入板内表面的生长试剂。 1.1.2 检测是基于比色技术的,在该技术中,当存在于水样中时,活的、好氧的、异养的、水性细菌会生长,显示出一种颜色反应,从而简化了菌落生长的可视化。 1.2 每个平板每1mL(CFU/1mL)样品可准确检测多达300个菌落形成单位。 为了增加定量范围,可以使用样品稀释。调整CFU/mL结果以反映稀释度。 1.3 该测试方法可用于饮用水(例如饮用水、瓶装水和牙科用水)和非饮用水(如冷却塔水)。用户有责任遵守当地法规的所有要求,并确保该测试方法对实验室间研究(ILS)中测试的水域以外的水域的有效性。 1.4 以国际单位制表示的数值应视为标准。本标准中不包括其他计量单位。 1.5 本标准并非旨在解决与其使用相关的所有安全问题(如有)。 本标准的使用者有责任在使用前制定适当的安全、健康和环境实践,并确定监管限制的适用性。 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ===意义和用途====== 5.1 这种平板形式可用于饮用水和非饮用水中可培养的水传播细菌的常规监测。 发现这些细菌的重要性可以帮助识别水质或水系统问题,或评估是否遵守维护协议。该测试方法使用小体积的水或其稀释液,提供了一种简单可靠的方法,可以消除培养基制备并减少实验室浪费。
1.1 This test method describes a simple procedure for the quantification of culturable, waterborne bacteria in potable water (drinking water, bottled water, and dental water, for example) and non-potable waters (cooling towers, for example). 1.1.1 The EasyDisc 2, 3 plate format is designed to test 1 mL of a water sample on a 47 mm gridded plate containing a growth reagent embedded to the plate’s inner surface. 1.1.2 Detection is based on colorimetric technology in which viable, aerobic, heterotrophic, waterborne bacteria grow when present in the water sample, displaying a color reaction which allows for a simplified visualization of colony growth. 1.2 Each plate can accurately detect up to 300 colony forming units per 1 mL (CFU/1 mL) of sample. To increase the quantification range, a sample dilution can be used. Adjust the CFU/mL result to reflect dilutions. 1.3 This test method can be used for potable (for example, drinking, bottled, and dental) waters and non-potable waters such as cooling tower waters. It is the user’s responsibility to adhere to all requirements by local regulations and ensure the validity of this test method for waters other than those tested as part of the Interlaboratory Study (ILS). 1.4 The values stated in SI units are to be regarded as the standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This plate format is useful for the routine monitoring of culturable, waterborne bacteria in potable and non-potable waters. The significance of finding these bacteria can help with identifying water quality or water system problems or evaluate compliance with maintenance protocols. This test method uses small volumes of water, or dilutions thereof, and provides an easy and reliable method that eliminates media preparation and reduces laboratory waste.
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归口单位: D19.24
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