Animal feeding stuffs — Determination of monensin, narasin and salinomycin contents — Liquid chromatographic method using post-column derivatization
动物饲料——莫能菌素、纳拉辛和盐霉素含量的测定——柱后衍生液相色谱法
发布日期:
2005-11-04
ISO 14183:2005规定了一种高效液相色谱(HPLC)方法,用于测定动物饲料、补充剂(干的和液体的)和矿物预混料中莫能菌素、纳拉辛和盐霉素的含量。本方法不适用于药物预混料(药品)。此方法无法测定拉沙洛西和塞姆杜拉米星。
莫能菌素、盐霉素和纳拉辛的定量限分别约为1 mg/kg、2 mg/kg和2 mg/kg。定量下限是可以实现的,但这需要用户验证。
ISO 14183:2005 specifies a high-performance liquid chromatographic (HPLC) method for the determination of the monensin, narasin and salinomycin contents of animal feeding stuffs, supplements (dry and liquid) and mineral premixtures. The method is not applicable to drug premixes (pharmaceutical products). Lasalocid and semduramicin cannot be determined by this method.
The limit of quantitation is approximately 1 mg/kg, 2 mg/kg and 2 mg/kg for monensin, salinomycin and narasin, respectively. A lower limit of quantitation can be achievable but this is to be validated by the user.