许多以前未被确认为食源性或有害的微生物正在出现 通过食物和水传播的人类病原体。新分类的Arcobacter 弯曲杆菌物种，最近被证明是一种新出现的病原体（Phillips， 2001）家畜和人类以及可能通过水传播的途径 还有食物。这项研究的目的是确定布茨勒里弧菌是否可以 在氯水处理后存活下来，并研究这种生物在水中的生存策略 氯化和非氯化饮用水。进行了两个存活实验 在含有100毫升水微观结构（氯化和非氯化）的烧瓶中进行- 氯化）通过0.2微米孔径和a.butzleri NCTC 12481细胞过滤。这个 烧瓶储存在12摄氏度的黑暗中。立即无菌取出样本 接种后（至），然后在接下来的2天内定期（氯化 饮用水）和接种35天（非氯化饮用水）。这个 实验是重复进行的。膜完整性（Baclight） 试剂盒），16S rRNA（FISH技术和EUB 338探针），DNA（PCR和23S rRNA PCR- RFLPs）和butzleri细胞可培养性（不含抗生素的TS琼脂平板）的变化 进行了分析。结果表明，A。 butzleri细胞在5分钟时丢失 在含有0.96 mg/l游离氯的氯化饮用水中。24小时后，细胞显示 膜损伤。24小时后，A。 检测到butzleri（2500 bp），但此时条带强度降低。这个 在氯气处理期间，16S rRNA的水平保持不变，因此可以杀死细菌 氯可能不涉及核糖体降解。这种生物能够 在不含氯的饮用水中存活至少2周，并能继续存活 在很长一段时间内。发现布兹列里菌对氯敏感 灭活。 饮用水处理中通常采用的消毒方法将是 足以控制这种有机体，但当 消毒是阻止传染源通过病毒传播的唯一障碍 Rice等人（1999年）推荐的受污染水源。 包括20个参考文献、表格和图表。

Many microorganisms previously unrecognized as food-borne or harmful are emerging as human pathogens transmitted by food and water. Arcobacter, the newly reclassified Campylobacter species, has been shown recently like an emerging pathogen (Phillips, 2001) of farm animals and human as well as possible routes of transmission via water and food. The objective of this work was to determine whether Arcobacter butzleri can survive chlorine water treatment and to study the survival strategies of this organism in chlorinated and non-chlorinated drinking water. Two survival experiments were conducted in flasks containing 100 ml water microcosms (chlorinated and non- chlorinated) filtered through a 0.2 um pore size and A. butzleri NCTC 12481 cells. The flasks were stored at 12C, in the dark. Samples were removed aseptically immediately after inoculation (To) and then periodically during the following 2 days (chlorinated drinking water) and 35 days (non-chlorinated drinking water) of inoculation. The experiments were performed by duplicate. The membrane integrity (Baclight viability kit), 16S rRNA (FISH technique and EUB 338 probe), DNA (PCR and 23S rRNA PCR- RFLPs) and culturability (TS agar plates without antibiotics) changes of A. butzleri cells were analyzed. The data showed that culturability of A. butzleri cells was lost at 5 min in chlorinated drinking water with 0.96 mg/l of free chlorine. At 24 h the cells showed membrane damage. After 24 h the amplicon specific for the 23S rRNA gene for A. butzleri (2500 bp) was detected, but the intensity of bands decreased at this time. The levels of 16S rRNA were constant during the chlorine treatment, so killing of bacteria with chlorine probably does not involve ribosome degradation. The organism was able to survive in non-chlorinated drinking water for at least 2 weeks and can remain viable for an extended period of time. A. butzleri was found to be sensitive to chlorine inactivation. Disinfection practices normally used in drinking water treatment would be adequate for controlling this organism, but continuous chlorination is essential when disinfection represents the only barrier to the spread of infectious agents via a contaminated water source, as recommended Rice et al. (1999). Includes 20 references, tables, figures.