本研究考察了预处理样品对聚合酶链反应（PCR）模板DNA回收的影响，以及卵囊数量与阈值周期之间的关系。卵囊的定量基于双标记荧光探针的使用和荧光强度的检测（以CT值测量）。本研究中使用的卵囊是从一头两周大的小牛的粪便中回收的，并通过免疫磁性分离方法进行纯化。使用市售试剂进行PCR。使用了两个已报道的引物和两个新设计的引物。在本实验中，用碱和蛋白酶K处理的样品显示出最低的CT值。在灵敏度方面，这种实时定量PCR方法检测到的卵囊只有一个。 卵囊数量与Ct值之间存在良好的相关性。进行该试验所需的总时间约为6.5小时（预处理为3.5小时，PCR为3小时）。包括4个参考文献、表格和图表。

This study examines the effect of pretreatment samples upon the recovery of DNA used for the template for polymerase chain reaction (PCR) and the relationship between the number of oocysts and the threshold cycle. The quantitation of oocysts was based on the use of dual-labeled fluorogenicprobe and the detection of fluorescent light intensity (measured as CT values). The oocysts used in this study were recovered from feces of a two week old calf and were purified by the immunomagnetic separation method. PCR was performed with the use of commercially available reagents. Two reported primers and two newly designed primers were used. In this experiment, samples treated with both alkali and protenase K showed the lowest CT value. With regard to the sensitivity, as low as one oocyst was detected by this real time quantitative PCR method. A good correlation between the number of oocysts and Ct values was observed. The total time required to perform this test was approximately 6.5 hours (3.5 hours for pretreatment and 3 hours for PCR). Includes 4 references, tables, figures.