本研究的目的是:传达服务实验室如何为美国环境保护局的方法1622草案制定标准操作程序:通过过滤/IMS/FA在水中产生隐孢子虫，以下简称方法1622；并协助供应商、工程师、实验室和监管机构识别任何成功检测原生动物的方法的基本组成部分。该实验室在数百个不同水源上实施了信息收集规则原生动物检测方法（ICR方法），并对方法1622进行了验证。ICR方法是美国使用最广泛的方法。 方法1622作为一种草案方法出版，许多验证实验继续进行，以评估其准确性和精密度。这两种方法都有几个组成部分:取样、颗粒提取、选择性分离、染色、鉴定和质量控制。此外，还采用ICR法和1622法从DI、原水和成品水中回收隐孢子虫。使用不连续蔗糖和氯化铯离心梯度纯化卵囊。使用1M蔗糖梯度和Percoll（R）1.05和1.09比重的阶梯梯度纯化蓝氏贾第鞭毛虫囊肿。囊肿储存在由500000单位青霉素、500000微克链霉素和50微克庆大霉素组成的抗生素溶液中，并在一个月内使用。

The objectives of this study are: to convey how a service laboratory is generating standard operating procedures for the US Environmental Protection Agency's draft Method 1622: Cryptosporidium in Water by Filtration/IMS/FA, hereafter Method 1622; and, to assist purveyors, engineers, laboratories and regulators in identifying essential components of any method for successful protozoan detection. The laboratory has performed the Information Collection Rule Protozoan Detection Method (ICR Method) on hundreds of different water sources as well as performing work on validation of Method 1622. The ICR method is the most widely used method in the United States. Method 1622 is published as a draft method and numerous validation experiments continue to be performed to assess its accuracy and precision. Both methods have several components: sampling, particulate extraction, selective separation, staining, identification, and quality control. Also, recoveries of Cryptosporidium from DI, raw and finished water using ICR and 1622 methods were made. Oocysts were purified using discontinuous sucrose and cesium chloride centrifugation gradients. Giardia lamblia cysts were purified using a 1M sucrose gradient followed by a step gradient of Percoll(R) 1.05 and 1.09 specific gravity. Cysts were stored in an antibiotic solution composed of 500,000 units Penicillin, 500,000 ug Streptomycin, and 50 ug Gentomycin, and used within a month.