Two commercially available defined-substrate coliform tests were compared with EC medium plus 4-methylumbelliferyl-B-D- Glucuronide (MUG) for detecting chlorine-exposed Escherichia coli in spiked water samples. Statistical analyses of the test results indicated no significant differences in detection of E. coli between the Autoanalysis Colilert test and EC medium with MUG. There were, however, significant differences in detection of E. coli between the ColiQuik test and EC medium with MUG in the free-chlorine-exposed pure culture studies and when all the data were combined. All methods were capable of detecting 1 cfu/100 mL of E. coli. Includes 41 references, tables.