本powerpoint演示文稿首先简要概述了生物高氯酸盐还原 以及用于治疗过程的分子生物学工具（MBT）。研究目标包括:开发定量分析靶向性 高氯酸盐还原基因； 检查高氯酸盐之间的关系 去除和基因表达水平；和 将qPCR和RT-qPCR分析应用于 高氯酸盐处理生物反应器。研究任务包括: 蛋白质定量分子分析方法的发展 高氯酸盐处理；和 分析在生物反应器中的应用。结论表明:qPCR和RT-qPCR检测提供了一种额外的方法 微生物高氯酸盐减少的证据 在生物反应器中发生； cld基因拷贝数通常较高 高氯酸盐去除率较高时； cld基因转录本拷贝数通常为 高氯酸盐去除率越高，去除率越高；当生物反应器性能下降时，基因 拷贝数和基因转录本拷贝数也有所下降；而且，至少可以解释生物反应器的故障 部分原因是微生物群落的变化。包括数字。

This powerpoint presentation begins by providing a brief overview of biological perchlorate reduction and molecular biology tools (MBTs) for treatment processes. Research objectives included: develop quantitative assay targeting perchlorate-reduction gene; examine relationship between perchlorate removal and gene expression levels; and, apply qPCR and RT-qPCR assays to perchlorate treatment bioreactor. Research tasks included: development of quantitative molecular assays for perchlorate treatment; and, application of assays to a bioreactor. Conclusions indicated that: qPCR and RT-qPCR assays provide an additional line of evidence that microbial perchlorate reduction was occurring in the bioreactor; cld gene copy numbers generally were higher when perchlorate removal was higher; cld gene transcript copy numbers generally were higher when perchlorate removal was higher; when bioreactor performance declined, gene copies and gene transcript copies also declined; and, bioreactor failure may be explained, at least in part, by changes in the microbial community. Includes figures.