本国际标准规定了生成和分析食源性细菌全基因组序列（WGS）数据的最低要求。这些要求适用于任何测序平台或化学。该过程可能包括以下阶段: •处理细菌培养物； •基因组DNA分离； •测序文库准备、测序和评估原始DNA序列读取质量和存储； •生物信息学分析，包括高质量单核苷酸多态性（hqSNP）等方法 分析、核心基因组和全基因组多位点基因分型（cgMLST、wgMLST）和生物信息管道验证； 和 •元数据捕获和序列存储库存储。

This document specifies the minimum requirements for generating and analysing whole genome sequencing (WGS) data of bacteria obtained from the food chain. This process can include the following stages: a) handling of bacterial cultures; b) axenic genomic DNA isolation; c) library preparation, sequencing, and assessment of raw DNA sequence read quality and storage; d) bioinformatics analysis for determining genetic relatedness, genetic content and predicting phenotype, and bioinformatics pipeline validation; e) metadata capture and sequence repository deposition; f) validation of the end-to-end WGS workflow (fit for purpose for intended application). This document is applicable to bacteria isolated from: —    products intended for human consumption; —    products intended for animal feed; —    environmental samples from food and feed handling and production areas; —    samples from the primary production stage.