冬季，在加拿大渥太华的不列颠净水厂进行了中试规模的实验。此外，还进行了使用水源水（加拿大安大略省渥太华河水）和缓冲Milli-Q水的实验室规模试验。数据显示，枯草芽孢杆菌孢子比隐孢子虫卵囊更容易受到二氧化氯灭活的影响1.4倍。因此，枯草芽孢杆菌孢子可能被视为隐孢子虫的替代品，因为它们也耐消毒，产生类似的失活水平。与过滤相关的物理剪切应力的影响似乎削弱了孢子，使其比“非”孢子更容易被二氧化氯灭活1.7倍- 扫描电子显微镜显示，过滤似乎会损坏孢子表面，从而增强微生物失活。因此，为了达到理想的微生物失活水平，与“非应激”孢子相比，需要较少的二氧化氯使“应激”孢子失活“孢子，最终可能会形成较少的DBP。正在进行的工作是将压力孢子的数据与受到相同剪切应力的隐孢子虫卵囊进行比较。包括14篇参考文献、表格和图表。

Pilot-scale experiments have been conducted during the winter months at the Britannia Water Purification Plant in Ottawa, Canada. In addition, bench-scale experiments using the source water (Ottawa River water Ontario, Canada) as well as buffered Milli-Q water have been conducted. The data have shown that Bacillus subtilis spores were 1.4 times more susceptible to chlorine dioxide inactivation than Cryptosporidium oocysts. Consequently, Bacillus subtilis spores may be considered as a possible surrogate for Cryptosporidium since they are also resistant to disinfection and yield similar inactivation levels. The impact of physical shearing stress that may be associated with filtration appears to weaken the spores and render them 1.7 times more susceptible to chlorine dioxide inactivation when compared to "non-stressed" spores. Scanning electron microscopy has revealed that filtration appears to damage the surface of the spores and consequently enhances microbial inactivation. Thus, to achieve a desired microbial inactivation level, less chlorine dioxide is required to inactivate "stressed" spores when compared to "non-stressed" spores, and, ultimately, less DBPS may be formed. Work is in progress to compare the data on stressed spores to Cryptosporidium oocysts that have been subjected to the same shearing stress. Includes 14 references, tables, figures.