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现行 ISO 19090:2018
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Tissue-engineered medical products — Bioactive ceramics — Method to measure cell migration in porous materials 组织工程医疗产品 - 生物活性陶瓷 - 多孔体的细胞迁移能力测试
发布日期: 2018-01-17
ISO 19090:2018规定了测量和记录多孔生物活性陶瓷材料的细胞迁移能力所需遵循的试验方法。 ISO 19090:2018不适用于具有低或无细胞粘附特性的多孔材料,例如合成聚合物和金属。这些类型的材料需要更长的时间才能使细胞从培养基质有效转移和迁移到试样。尽量减少细胞传代、细胞种类、细胞培养耗材(包括培养基和胎牛血清)差异的影响。 ,该方法使用各国临床上广泛使用的多孔生物活性陶瓷作为计算相对迁移距离的参考材料。
ISO 19090:2018 specifies the test method to be followed for measuring and documenting the cell migration ability of porous bioactive ceramic materials. ISO 19090:2018 is not applicable to porous materials that have low or no cell adhesion properties, for instance synthetic polymers and metals. These types of materials will require longer times to allow effective transfer and migration of cells from the cultured substrate to the test specimen. To minimize influences of cell passages, cell kinds, differences in cell culture consumables including culture medium and fetal bovine serum etc., the method uses a porous bioactive ceramics, which is clinically and widely used in each country, as a reference material for calculation of relative migration distance.
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归口单位: ISO/TC 150/SC 7
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