1.1 本规程概述了用于控制冷却水系统中微生物生长的杀微生物剂（杀藻剂、杀菌剂和杀菌剂）的功效评估程序。使用模拟或真实的冷却塔水对杀菌剂进行评估( 1. )冷却水中的微生物( 2. )运行冷却系统微生物沉积物（生物膜）中的微生物，或( 3. )已知污染冷却水系统的微生物或其组合。这种做法应由熟悉微生物学技术的人员进行。 1.2 以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.3 本标准并非旨在解决与其使用相关的所有安全问题（如有）。 本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.4 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 本规程确定了使用现场获得的冷却水和沉积物/生物膜在冷却水系统中使用的潜在有效杀微生物剂。沉积物/生物膜的添加解决了冷却水系统中微生物主要来源的需要。 即使增加了这一点，实验室结果也可能无法完全预测现场的杀菌效果。这是因为影响杀微生物剂有效性的现场条件很难在实验室模拟。影响杀微生物剂效果的条件包括排污率、补充水的添加、水硬度、碳氢化合物泄漏、pH值、沉积物含量、溶解固体、粘液（生物膜）中的微生物以及表面上的沉积物（盐、铁矿物、有机物等）。另一个因素是，由于缺乏足够的回收介质，难以枚举水中的所有微生物。关于表面附着微生物（生物膜）的形成和测试的指南可在指南中找到 E1427 ，而非常规微生物测量指南见指南 E1326 .

1.1 This practice outlines a procedure for evaluating the efficacy of microbicides (algicides, bactericides, and fungicides) that will be used for controlling microbial growth in cooling water systems. The microbicides will be evaluated using simulated or real cooling tower water against ( 1 ) microbes from cooling water, ( 2 ) microbes in microbiological deposits (biofilms) from operating cooling systems, or ( 3 ) microorganisms known to contaminate cooling water systems, or a combination thereof. This practice should be performed by individuals familiar with microbiological techniques. 1.2 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.3 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.4 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 This practice determines potentially effective microbicides for use in cooling water systems using cooling water and deposits/biofilm obtained from the field. The addition of deposits/biofilms addresses the need to include the major source of microorganisms in cooling water systems. Even with this addition, laboratory results may not be totally predictive of microbicidal effectiveness in the field. This is because conditions in the field affecting microbicide effectiveness are difficult to mimic in the laboratory. These conditions that affect microbicide efficacy include blow-down rate, addition of makeup water, water hardness, hydrocarbon leaks, pH, sediment loading, dissolved solids, microbes in slime (biofilms), and deposits (salts, iron minerals, organics, and so forth) on surfaces. An additional factor is the difficulty in enumerating all microbes in the water due to the lack of adequate recovery media. Guidelines that address formation of and testing for surface-attached microbes (biofilms) may be found in Guide E1427 , while a guideline for unconventional measurement of microbes is found in Guide E1326 .