ISO 21150:2015给出了化妆品中特定微生物大肠杆菌检测和鉴定的一般指南。根据国家实践或法规，本国际标准中规定的微生物可能因国家而异。 为了确保消费者的产品质量和安全，建议进行适当的微生物风险分析，以确定本国际标准适用的化妆品类型。被认为具有低微生物风险（见ISO 29621）的产品包括水活性低的产品，如hydro- 酒精产品、极端pH值等。 本国际标准中描述的方法基于在非选择性液体培养基（浓缩肉汤）中检测大肠杆菌，然后在选择性琼脂培养基上分离。根据所需的检测水平，其他方法可能是合适的。 注:为了检测大肠杆菌，可以在非选择性培养基上进行继代培养，然后进行适当的鉴定步骤（例如使用鉴定试剂盒）。 由于该应用领域内的化妆品种类繁多，这种方法可能不适用于某些产品的每个细节（例如:。 g、 某些水不溶性产品）。其他国际标准（ISO 18415）可能适用。如果证明了其他方法的等效性，或者证明了该方法的适用性，则可以用其他方法（如自动方法）代替本文所述的试验。

ISO 21150:2015 gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc. The method described in this International Standard is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate, depending on the level of detection required. NOTE For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits). Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) can be substituted for the test presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.