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The Best Way to Detect Viruses in Water Samples 检测水样中病毒的最佳方法
发布日期: 2005-11-01
本研究的目的是开发一种检测肠道病毒的方法 不同种类的水样,采用适合检测传染病的浓缩法 病毒以及病毒RNA。五种从水中浓缩肠道病毒的方法和一种RNA 从参考文献中选择提取物或在实验室中开发,然后进行比较 通过逆转录酶PCR(RT-PCR)对一个PBS加标样本进行评估。在预赛期间 通过分析发现,受试浓度方案之间存在效率差异。包括46个参考文献和表格。
The objective of this study was to develop a method for the detection of a large range of enteric viruses in different kinds of water samples, using a concentration method suitable for the detection of infectious viruses as well as virus RNA. Five methods of concentration of enteric viruses from water and one RNA extraction were selected from the bibliography or developed in a laboratory and then were comparatively evaluated by reverse transcriptase PCR (RT-PCR) on one PBS spiked sample. During those preliminary assays, efficiency differences were noticed between the concentration protocols tested. Includes 46 references, tables.
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发布单位或类别: 美国-美国给水工程协会
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