1.1本规程提供了获得指定为X、Y、Z和X的三刺激值所需的值和实际计算程序 10 Y 10 Z 10 对于CIE 1931和1964观察员，分别来自样本的双谱光度数据。获取此类双谱光度数据的程序包含在实践中 E2153 . 1.2将结果转换为作为CIE系统一部分的颜色空间（如CIELAB和CIELUV）的程序包含在实践中 E308年 . 1.3 本标准可能涉及危险材料、操作和设备。 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。 ====意义和用途====== 双谱或双单色仪法是测定荧光样品一般辐射传输特性的确定方法 (4) . 在这种方法中，测量仪器配备了两个单独的单色器。 第一种是辐照单色仪，用单色光照射试样。第二个是观察单色仪，分析离开样品的辐射。通过将辐照单色仪设置在一系列固定波长处，获得了双谱光度值的二维阵列 ( μ ) 在紫外线和可见光范围内 μ ，使用观察单色仪记录每个波长的读数 ( λ ) 在可见范围内。一旦正确校正，得到的阵列称为Donaldson矩阵，以及每个元素的值 ( μ , λ ) 该阵列的辐射因子在这里称为Donaldson辐射因子 （D）( μ , λ )) . Donaldson辐射因子是与仪器和光源无关的样本光度特性，可用于计算任何所需光源和观察者的颜色。该方法的优点是，它提供了样本辐射传输特性的全面表征，而不存在与源模拟和各种近似方法相关的不精确性。

1.1 This practice provides the values and practical computation procedures needed to obtain tristimulus values, designated X, Y, Z and X 10 , Y 10 , Z 10 for the CIE 1931 and 1964 observers, respectively, from bispectral photometric data for the specimen. Procedures for obtaining such bispectral photometric data are contained in Practice E2153 . 1.2 Procedures for conversion of results to color spaces that are part of the CIE system, such as CIELAB and CIELUV are contained in Practice E308 . 1.3 This standard may involve hazardous materials, operations, and equipment. This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. ====== Significance And Use ====== The bispectral or two-monochromator method is the definitive method for the determination of the general radiation-transfer properties of fluorescent specimens (4) . In this method, the measuring instrument is equipped with two separate monochromators. The first, the irradiation monochromator, irradiates the specimen with monochromatic light. The second, the viewing monochromator, analyzes the radiation leaving the specimen. A two-dimensional array of bispectral photometric values is obtained by setting the irradiation monochromator at a series of fixed wavelengths ( μ ) in the ultraviolet and visible range, and for each μ , using the viewing monochromator to record readings for each wavelength ( λ ) in the visible range. The resulting array, once properly corrected, is known as the Donaldson matrix, and the value of each element ( μ , λ ) of this array is here described as the Donaldson radiance factor (D( μ , λ )) . The Donaldson radiance factor is an instrument- and illuminant-independent photometric property of the specimen, and can be used to calculate its color for any desired illuminant and observer. The advantage of this method is that it provides a comprehensive characterization of the specimen's radiation-transfer properties, without the inaccuracies associated with source simulation and various methods of approximation.