1.1 本试验方法涵盖了用于检测和计数水中肠球菌的膜过滤器（MF）程序。肠球菌，包括 粪肠球菌 ( E、 粪便 ), E、 粪便 它们的变种通常存在于人类和其他温血动物的粪便中。虽然有些菌株普遍存在，与粪便污染无关，但水中的肠球菌表明粪便污染和可能存在肠道病原体。这些细菌在水和废水中的密度范围很广。检测限为一个菌落形成单位（CFU）/过滤体积。 1.2 该试验方法已成功用于温带淡水和海洋环境水以及废水。用户有责任确保本试验方法对未经试验类型的水的有效性。 1.3 以国际单位制表示的数值应视为标准值。 国际单位制后括号中给出的值仅供参考，不被视为标准值。 1.4 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 有关具体的危险说明，请参阅第节 9 . 1.5 本国际标准是根据世界贸易组织技术性贸易壁垒（TBT）委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认标准化原则制定的。 ====意义和用途====== 5.1 肠球菌是饮用水、贝类养殖水、环境和娱乐用水的细菌学质量指标。 通过流行病学研究以及海洋和淡水浴场，已经确定了游泳、相关胃肠炎和肠球菌之间的直接关系。这些研究导致了标准的发展，可用于根据已建立的健康水质关系制定洗澡水标准。 5.2 由于可以通过膜过滤技术分析少量或大量的水或其稀释液，因此可以列举和检测水中各种程度的肠球菌。

1.1 This test method covers a membrane filter (MF) procedure for the detection and enumeration of the enterococci bacteria in water. The enterococci, which include Entero-coccus faecalis ( E. faecalis ), E. faecium , and their varieties are commonly found in the feces of humans and other warm-blooded animals. Although some strains are ubiquitous and not related to fecal pollution, enterococci in water are an indication of fecal pollution and the possible presence of enteric pathogens. These bacteria are found in water and wastewater in a wide range of densities. The detection limit is one colony forming unit (CFU)/volume filtered. 1.2 This test method has been used successfully with temperate fresh and marine ambient waters, and wastewaters. It is the user’s responsibility to ensure the validity of this test method for waters of untested types. 1.3 The values stated in SI units are to be regarded as standard. The values given in parentheses after SI units are included for information only and are not considered standard. 1.4 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 9 . 1.5 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 The enterococci are indicators of the bacteriological quality for potable water, shellfish growing waters, ambient, and recreational waters. A direct relationship between swimming, associated gastroenteritis, and enterococci has been established through epidemiological studies and marine and fresh water bathing beaches. These studies have led to the development of criteria that can be used to establish bathing water standards based on established health-water quality relationships. 5.2 Since small or large volumes of water or dilutions thereof, can be analyzed by the membrane filter technique, a wide range of levels of enterococci in water can be enumerated and detected.