1.1 本程序包括通过使用液相色谱法（LC）直接注射测定地表水中的甲基膦酸二异丙酯（DIMP）、二甲酰胺磷酸氢乙酯（EHDMAP）、甲基膦酸乙酯（EMPA）、甲基磷酸异丙酯（IMPA）、甲基膦酸甲酯（MPA）和频哪醇基甲基膦酸（PMPA）（在本试验方法中统称为有机膦酸盐），并使用电喷雾电离法（ESI）用串联质谱法（MS/MS）检测。这些分析物通过该试验方法进行定性和定量测定。该测试方法遵循单反应监测（SRM）质谱法。 1.2 该测试方法由美国环保局芝加哥地区实验室（CRL）开发。 1.3 以国际单位制表示的数值应视为标准。本标准不包括其他计量单位。 1.4 有机膦酸盐的检测验证水平（DVL）和报告范围列于 表1 . 1.4.1 DVL的浓度要求至少比报告限值（RL）低三倍，且信噪比大于3:1。 图1 显示ESI正模式下有机膦酸酯DVL的信噪比，以及 图2 在ESI负模式下。 1.5 本标准并不旨在解决与其使用相关的所有安全问题（如有）。本标准的使用者有责任在使用前制定适当的安全、健康和环境实践，并确定监管限制的适用性。 1.6 本国际标准是根据世界贸易组织技术性贸易壁垒委员会发布的《关于制定国际标准、指南和建议的原则的决定》中确立的国际公认的标准化原则制定的。 ====意义和用途====== 5.1 有机磷农药通过破坏调节乙酰胆碱（一种神经递质）的酶来影响神经系统。它们是在19世纪初开发的，但它们对昆虫的影响与对人类的影响相似，是在1932年发现的。有些是有毒的，被用作化学武器制剂。有机磷农药通常在环境中不持久。 5. , 6. 5.2 本试验方法用于分析Sarin（GB）、Soman（GD）、Tabun（GA）和VX中选定的有机磷基化学武器剂降解产物。 该测试方法已被研究用于试剂和地表水。

1.1 This procedure covers the determination of diisopropyl methylphosphonate (DIMP), ethyl hydrogen dimethylamidophosphate (EHDMAP), ethyl methylphosphonic acid (EMPA), isopropyl methylphosphonic acid (IMPA), methylphosphonic acid (MPA) and pinacolyl methylphosphonic acid (PMPA) (referred to collectively as organophosphonates in this test method) in surface water by direct injection using liquid chromatography (LC) and detected with tandem mass spectrometry (MS/MS) using electrospray ionization (ESI). These analytes are qualitatively and quantitatively determined by this test method. This test method adheres to single reaction monitoring (SRM) mass spectrometry. 1.2 This test method has been developed by U.S. EPA Region 5 Chicago Regional Laboratory (CRL). 1.3 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.4 The detection verification level (DVL) and reporting range for the organophosphonates are listed in Table 1 . 1.4.1 The DVL is required to be at a concentration at least three times below the reporting limit (RL) and have a signal/noise ratio greater than 3:1. Fig. 1 displays the signal/noise ratios at the DVLs for the organophosphonates in the ESI positive mode and Fig. 2 in the ESI negative mode. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. ====== Significance And Use ====== 5.1 Organophosphate pesticides affect the nervous system by disrupting the enzyme that regulates acetylcholine, a neurotransmitter. They were developed during the early 19th century, but their effects on insects, which are similar to their effects on humans, were discovered in 1932. Some are poisonous and were used as chemical weapon agents. Organophosphate pesticides are usually not persistent in the environment. 5 , 6 5.2 This test method is for the analysis of selected organophosphorous-based chemical weapon agent degradation products from Sarin (GB), Soman (GD), Tabun (GA) and VX. This test method has been investigated for use with reagent and surface water.