Ambient air. Monitoring the effects of genetically modified organisms (GMO). Pollen monitoring-Technical pollen sampling using pollen mass filter (PMF) and Sigma-2-sampler
环境空气 监测转基因生物(GMO)的影响 花粉监测
BS PD CEN/TS 16817-1:2015 describes a procedure for the use of the passive samplers Sigma-2 and PMF
to sample airborne pollen. Both are designed to sample coarse aerosol particles. Collected samples are
used to analyse pollen input with regard to pollen type and amount, and input of transgenic pollen. The
Sigma-2 passive sampler here provides a standardized sampling method for direct microscopic pollen
analysis and quantifying the input of airborne pollen at the site. The PMF yields sufficient amounts of
pollen to additionally carry out molecular-biological diagnostics for detection of GMO.Essential background information on performing GMO monitoring is given in VDI/Guideline 4330
Part 1 [4], which is based on an integrated assessment of temporal and spatial variation of GMO
cultivation (sources of GMO), the exposure in the environment and biological/ecological effects. Ideally,
the pollen sampling using technical samplers for GMO monitoring should be undertaken in combination
with the biological collection of pollen by bees (CEN/TS 16817-2).The application of technical passive samplers and the use of honey bee colonies as active biological
collectors complement each other in a manifold way when monitoring the exposure to GMO pollen.
Technical samplers provide results regarding the pollen input at the sampling site in a representative
way, whereas with biological sampling by honey bee colonies, pollen from flowering plants in the area
is collected according to the bees' collection activity. Thus, this method represents GMO exposure to
roaming insects. By combining the two sampling methods these two main principles of exposure are
represented. Furthermore, a broad range of pollen species is covered.The sample design depends on the intended sampling objective. Some examples are given in 6.2.Cross References:VDI 2119:2013-06ISO 5725-1DIN 10760DIN 32645VDI 4330 Part 12001/18/EC2002/811/ECCEN/TS 16817-2VDI 4330 Part 3VDI 4330 Part 4All current amendments available at time of purchase are included with the purchase of this document.