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现行 BS PD CEN/TS 16817-1:2015
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Ambient air. Monitoring the effects of genetically modified organisms (GMO). Pollen monitoring-Technical pollen sampling using pollen mass filter (PMF) and Sigma-2-sampler 环境空气 监测转基因生物(GMO)的影响 花粉监测
发布日期: 2015-10-31
BS PD CEN/TS 16817-1:2015描述了被动取样器Sigma-2和PMF的使用程序 采集空气中的花粉。这两种方法都是为了对粗糙的气溶胶颗粒进行采样而设计的。收集的样本包括 用于分析花粉输入的类型和数量,以及转基因花粉的输入。这个 Sigma-2被动采样器为直接显微花粉提供了一种标准化的采样方法 分析和量化现场空气中花粉的输入。PMF产生足够数量的 花粉还需要进行分子生物学诊断以检测转基因。VDI/4330指南中给出了执行GMO监控的基本背景信息 第1部分[4],基于转基因作物时空变异的综合评估 种植(转基因来源)、环境暴露和生物/生态影响。理想的, 应使用转基因花粉采样器进行技术组合监测 通过蜜蜂生物收集花粉(CEN/TS 16817-2)。技术被动采样器的应用和蜜蜂群体作为主动生物的使用 在监测转基因花粉暴露时,采集者以多种方式相互补充。 技术采样器以代表性的方式提供有关取样点花粉输入的结果 然而,通过蜜蜂群落的生物取样,该地区开花植物的花粉 根据蜜蜂的采集活动进行采集。因此,这种方法代表了转基因生物的风险敞口 漫游的昆虫。通过结合这两种采样方法,这两个主要的暴露原则是 代表。此外,还涵盖了广泛的花粉种类。样本设计取决于预期的抽样目标。 6.2中给出了一些例子。交叉引用:VDI 2119:2013-06ISO 5725-1DIN 10760DIN 32645VDI 4330第12001/18/EC2002/811/ECCEN/TS 16817-2VDI 4330第3VDI 4330部分第4部分购买本文件时可获得的所有当前修改件均包含在购买本文件中。
BS PD CEN/TS 16817-1:2015 describes a procedure for the use of the passive samplers Sigma-2 and PMF to sample airborne pollen. Both are designed to sample coarse aerosol particles. Collected samples are used to analyse pollen input with regard to pollen type and amount, and input of transgenic pollen. The Sigma-2 passive sampler here provides a standardized sampling method for direct microscopic pollen analysis and quantifying the input of airborne pollen at the site. The PMF yields sufficient amounts of pollen to additionally carry out molecular-biological diagnostics for detection of GMO.Essential background information on performing GMO monitoring is given in VDI/Guideline 4330 Part 1 [4], which is based on an integrated assessment of temporal and spatial variation of GMO cultivation (sources of GMO), the exposure in the environment and biological/ecological effects. Ideally, the pollen sampling using technical samplers for GMO monitoring should be undertaken in combination with the biological collection of pollen by bees (CEN/TS 16817-2).The application of technical passive samplers and the use of honey bee colonies as active biological collectors complement each other in a manifold way when monitoring the exposure to GMO pollen. Technical samplers provide results regarding the pollen input at the sampling site in a representative way, whereas with biological sampling by honey bee colonies, pollen from flowering plants in the area is collected according to the bees' collection activity. Thus, this method represents GMO exposure to roaming insects. By combining the two sampling methods these two main principles of exposure are represented. Furthermore, a broad range of pollen species is covered.The sample design depends on the intended sampling objective. Some examples are given in 6.2.Cross References:VDI 2119:2013-06ISO 5725-1DIN 10760DIN 32645VDI 4330 Part 12001/18/EC2002/811/ECCEN/TS 16817-2VDI 4330 Part 3VDI 4330 Part 4All current amendments available at time of purchase are included with the purchase of this document.
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发布单位或类别: 英国-英国标准学会
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BS PD CEN/TS 16817-2-2015
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