ISO 21527-1:20 08规定了一种水平方法，用于计数用于人类消费或动物喂养的产品中的活酵母和霉菌，其水分活度大于0.95【鸡蛋、肉类、乳制品（奶粉除外）、水果、蔬菜、新鲜糊状物等。】，通过在25°C±1°C下的菌落计数技术。 ISO 21527-1:20 08不允许计数霉菌孢子。真菌菌群的鉴定和食品中真菌毒素的检查都不在ISO 21527-1:20 08的范围内。ISO 21527-1:20 08中规定的方法不适用于计数罐装或瓶装水果和蔬菜中的耐热真菌，如黄腐双孢菌或妮维雅双孢菌。

ISO 21527-1:2008 specifies a horizontal method for the enumeration of viable yeasts and moulds in products, intended for human consumption or feeding of animals, having a water activity greater than 0,95 [eggs, meat, dairy products (except milk powder), fruits, vegetables, fresh pastes, etc.], by means of the colony count technique at 25 °C plus or minus 1 °C. ISO 21527-1:2008 does not allow the enumeration of mould spores. Neither the identification of fungal flora nor the examination of foods for mycotoxins lie within the scope of ISO 21527-1:2008. The method specified in ISO 21527-1:2008 is not suitable for enumeration of heat-resistant fungi, such as Byssochlamys fulva or Byssochlamys nivea, in canned or bottled fruit and vegetables.