ISO 23893-3:2013规定了采用酶联免疫吸附试验（ELISA）方法测量鱼血浆样品中卵黄蛋白原（Vtg）浓度的方法。 它适用于在环境（淡水、河口或盐水）中取样的鱼类，以及在实验室中接触物质或废水的鱼类。当使用Vtg抗体和Vtg标准品时，该方法是定量的，并具有所选物种的特征。

ISO 23893-3:2013 specifies a method for measuring vitellogenin (Vtg) concentrations in a fish plasma sample employing an enzyme-linked immunosorbent assay (ELISA) method. It applies to fish that are sampled in the environment (fresh, estuarine or salt water) and to fish exposed to substances or effluents in a laboratory. The method is quantitative when using Vtg antibodies and a Vtg standard well characterized with the species of choice.