适用于变性淀粉，颗粒状和可溶于冷水。规定的方法能够测量总乙酰基和游离乙酰基含量；结合乙酰基含量计算为两个值的差值。通过用稀盐酸加热样品，并用5-三磷酸腺苷（ATP）和辅酶A（CoA）将醋酸盐转化为乙酰辅酶A，测定总含量。该方法适用于乙酰基含量高达2%（m/m）的测定。

Applies to modified starch, both granular and soluble in cold water. The methods specified enable to measure total and free acetyl contents; bound acetyl content is calculated as the difference of the two values. Total content is determined by heating the sample with dilute hydrochloric acid and converting the acetate with adenosine-5-triphosphate (ATP) and coenzyme A (CoA) to acetyl-CoA. The methods are suitable for determining acetyl contents up to 2 % (m/m).