ISO 4831:2006给出了大肠菌群检测和计数的一般指南。适用于人类食用和动物饲养的产品，以及食品生产和食品处理领域的环境样品。 在30°C或37°C的液体培养基中培养后，通过计算最可能数（MPN）进行计数。 当所寻求的数量预计在每毫升或每克试样1到100的范围内时，此枚举方法适用。

ISO 4831:2006 gives general guidelines for the detection and the enumeration of coliforms. It is applicable to products intended for human consumption and for the feeding of animals, and to environmental samples in the area of food production and food handling. Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37 °C. This enumeration method is applicable when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.