Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of presumptive Bacillus cereus — Colony-count technique at 30 degrees C
食品和动物饲料微生物学——推定蜡样芽孢杆菌计数的水平方法——30℃时菌落计数技术
发布日期:
2004-06-16
ISO 7932:2004规定了在30℃下通过菌落计数技术计数活蜡样芽孢杆菌的水平方法。它适用于人类消费和动物喂养的产品,以及食品生产和食品处理领域的环境样品。
为了有一个可行的测试方法,确认阶段被限制在MYP琼脂上的典型方面和溶血测试。因此,引入术语“推定”是为了承认这样一个事实,即确证阶段不能将蜡样芽孢杆菌与其他密切相关但不太常见的芽孢杆菌物种(如炭疽芽孢杆菌、苏云金芽孢杆菌、魏氏芽孢杆菌、分枝杆菌)区分开来。在怀疑存在蜡样芽孢杆菌的情况下,额外的运动性测试可能有助于区分蜡样芽孢杆菌和炭疽芽孢杆菌。
ISO 7932:2004 specifies a horizontal method for the enumeration of viable Bacillus cereus by means of the colony-count technique at 30 °C. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.
In order to have a practicable test method, the confirmatory stage has been restricted to the typical aspect on MYP agar and the haemolysis test. Thus the term presumptive has been introduced in order to acknowledge the fact that the confirmatory stage does not enable the distinction of B. cereus from other closely related but less commonly encountered Bacillus species, such as B. anthracis, B. thuringiensis, B. weihenstephanensis, B. mycoides. An additional motility test may help to differentiate B. cereus from B. anthracis in cases where the presence of the latter is suspected.