ISO 17512-1:2008规定了一种快速筛选方法，用于评估土壤的生境功能以及污染物和化学品对蚯蚓行为的影响。亚致死试验是一种快速的方法，它反映了天然土壤中污染物混合物和掺入土壤的物质对胎儿艾森氏菌和安德烈艾森氏菌的生物有效性。蠕虫的躲避行为是测试的测量终点。本试验并非旨在取代蚯蚓繁殖试验。两种不同的设计（两段式和六段式）已经开发并成功应用。这两种设计都适用于单一浓度（例如用于评估田间土壤的质量）或多浓度（例如用于评估加标化学品的毒性）试验。在这两种情况下，蚯蚓都可以对哪个隔间、控制和处理进行初步选择[在两段式试验容器中，在右侧和右侧之间左侧；在六节试验容器之间（3+3）交替隔室），进入。

ISO 17512-1:2008 specifies a rapid screening method for evaluating the habitat function of soils and the influence of contaminants and chemicals on earthworm behaviour. The sublethal test is a rapid method that reflects the bioavailability of contaminant mixtures in natural soils and substances spiked into soils to Eisenia fetida and Eisenia andrei. The avoidance behaviour of the worms is the measurement endpoint of the test. This test is not intended to replace the earthworm reproduction test. Two different designs (a two section unit and a six section unit) have been developed and successfully applied. Both designs are applicable to either single-concentration (e. g. for assessing the quality of a field soil) or multi-concentration (e. g. for assessing the toxicity of a spiked chemical) tests. In both cases, the earthworms are allowed to make the initial choice on which compartment, control and a treatment [in the two section test vessel between right and left side; in the six section test vessel between the (3 + 3) alternating compartments], to enter.