利用免疫荧光法（IFA）对环境样品中的隐孢子虫卵囊进行了大规模鉴定。由于IFA检测到所有隐孢子虫的卵囊，环境样本中隐孢子虫寄生虫的种类分布和来源尚不清楚。作者之前开发了一种基于小亚单位（SSU）rRNA的聚合酶链反应-限制性内切酶长度多态性（PCR-RFLP）技术，用于临床样本中隐孢子虫寄生虫的物种和菌株特异性诊断。在这项研究中，作者将这项技术与免疫磁性分离相结合，以确定水样中隐孢子虫卵囊的种类和来源。 用该技术对8次降雨后采集的29个风暴样本进行了测试。包括30个参考文献、表格、图表。

The identification of Cryptosporidium oocysts in environmental samples is largelymade by the use of immunofluorescent assay (IFA). Because IFA detects oocystsfrom all Cryptosporidium parasites, the species distribution and source ofCryptosporidium parasites in environmental samples is unclear. The authorspreviously developed a small subunit (SSU) rRNA-based polymerase chainreaction-restriction enzyme length polymorphism (PCR-RFLP) technique for species-and strain-specific diagnosis of Cryptosporidium parasites in clinical samples.In this study, the authors used this technique in conjunction with immunomagneticseparation to identify species and sources of Cryptosporidium oocysts present inwater samples. Twenty-nine storm samples collected after 8 rain events weretested with this technique. Includes 30 references, table, figures.