这项工作表明，基于HCT-8细胞培养的传染性与RT-PCR相结合 检测微小假丝酵母菌感染是一种实用的工具，可以提供有关微小假丝酵母菌感染的有价值信息 消毒剂的功效和环境水体中卵囊的传染性。这是一个广泛存在的问题 支持微小隐孢子虫两种主要基因型生长的适用技术 已被证明相当于小鼠传染性的金标准。结果是五分之一 分离株表明，细胞培养中的传染性是一个很好的指标，表明卵囊的抗感染能力 导致动物感染。然而，细胞系的选择和检测方法 感染对确定等效性很重要。在不同的情况下也是如此 小鼠模型，因为一些菌株对感染表现出不同的敏感性。紫外线消毒（UV）是一种 极有前途的消毒技术，可大大降低对公众健康的风险 由水传播隐孢子虫病引起。本报告中提供的消毒数据 证明HCT-8细胞培养/RT-PCR试验也相当于小鼠试验 测量微小隐球菌卵囊的紫外线灭活。因此，基于体外细胞培养的检测 应被视为使用动物来测量疾病传染性的实用替代方法 C.微小弧菌，并评估消毒剂的效力。然而，基于细胞培养的消毒 分析的设计必须具有足够的重复性和统计稳健性，以确保可信度 在结果中。感染性分析中的自然变异性，至少两种 不同寄主范围的微小隐孢子虫初级基因型及其剂量效应的变化 不同隔离体的曲线都表明，在进行试验时应采取谨慎的方法 解释细胞培养或动物灭活数据。未来基于细胞培养的灭活 研究应该更广泛地调查细胞培养和动物实验之间的相关性 消毒剂的范围（例如臭氧和二氧化氯）。此外，更多的细胞 每个数据点的单层需要成为实验设计的一部分，并具有更广泛的多样性 需要研究属于这两种主要基因型的菌株的数量。而且，应该有 努力使基于细胞培养的感染性分析标准化（例如，使用相同年龄的卵囊 并在相同条件下生产），以便在研究之间进行比较。在里面 除了标准化之外，未来的工作还应该包括对细胞培养中传染性的评估 比较了人类志愿者的感染情况，并对不同的方法进行了彻底比较 可用于检测和定量细胞培养中的感染。 包括28篇参考文献、图表。

This work demonstrated that an HCT-8 cell culture-based infectivity coupled with RT-PCR for detecting C. parvum infections is a practical tool that can provide valuable information about the efficacy of disinfectants and the infectivity of oocysts in environmental waters. It is a widely applicable technique that supports the growth of the two primary genotypes of C. parvum and has been shown to be equivalent to the gold standard of mouse infectivity. The results with five isolates demonstrated that infectivity in cell culture is a good indicator of the ability of oocysts to cause infection in animals. However, the choice of cell line and the method used to detect infection is important in determining equivalency. This would also be the case with different mouse models since some strains demonstrate different sensitivities to infection. Ultraviolet disinfection (UV) is an extremely promising disinfection technology which may greatly reduce the risk to public health posed by waterborne cryptosporidiosis. The disinfection data presented in this report demonstrates that the HCT-8 cell culture/RT-PCR assay was also equivalent to a mouse assay for measuring UV inactivation of C. parvum oocysts. Therefore, in-vitro cell culture-based assays should be regarded as practical alternatives to the use of animals for measuring the infectivity of C. parvum and assessing the efficacy of disinfectants. However, cell culture-based disinfection assays must be designed with sufficient replication and statistical robustness to allow confidence in the results. The natural variability within infectivity assays, the identification of at least two primary genotypes of C. parvum with different host ranges, and the variation in dose response curves for different isolates all suggest that a cautious approach should be adopted when interpreting cell culture or animal-based inactivation data. Future cell culture-based inactivation studies should investigate the correlation between cell culture and animal assays for a broader range of disinfectants (e.g., ozone and chlorine dioxide). In addition, a larger number of cell monolayers for each data point needs to be part of the experimental design and a wider diversity of isolates belonging to both of the primary genotypes needs to be studied. Also, there should be efforts to standardize cell culture-based infectivity assays (e.g., using oocysts of the same age and produced under the same conditions) so that comparisons can be made between studies. In addition to standardization, future work should include an evaluation of infectivity in cell culture compared to infection in human volunteers and a thorough comparison of the different methods available for detecting and quantitating infections in cell culture. Includes 28 references, figures.