长期以来，人们一直需要开发用于临床的自主病原体检测系统 水样将消除获取所需的时间、可变性和操作员参与 准确的结果。中国病原体自动检测系统的构建与验证 供水是一个必须考虑的重大挑战:代表性水量 必须对水进行取样和检查，以对结果具有统计可信度；这个 用于将样品浓缩成合理体积进行分析的方法；这个 检测所需的处理和纯化步骤；所选的检测平台（如电池） 培养、PCR、荧光抗体）；以及完成这些任务所需的工程 能够提供精确结果且假阳性和假阴性有限的任务 错误率。作者们目前正在测试一种最初设计的试验板现场设备 专为雾化生物恐怖剂设计。然而，推动 该系统可以很容易地用于水质监测。现场装置的原型是 目前正在为大肠杆菌O157:H7开发。该设备可以自动采集多达100个样本 毫升水。去除抑制剂的处理过程是通过流动来完成的 免疫磁分离。然后将固定在磁珠上的纯化细胞分离 转移到流动PCR系统，直接从珠子上进行PCR。这个 收集洗脱液，并将PCR样品与带有特定探针的阵列杂交 检测这些产品。自动化方案的特异性和敏感性都很高 杰出的在基质水（哥伦比亚河水）中持续回收10个加标细胞是必要的 常规实现。系统架构和PCR热循环协议 消除PCR残留，使系统在配管和其他操作之前可以多次使用 需要易于用户更换的部件。下一代系统将能够收集大量数据 更大体积的水（高达10升），以及针对多种试剂的高度多重PCR链接，以及 流动珠阵列系统，用于真正自主检测任何水性病原体。 包括31篇参考文献、图表。

There has been a long-standing need to develop autonomous pathogen detection systems for water samples that will eliminate the time, variability, and operator involvement needed to obtain accurate results. Construction and validation of an autonomous system for pathogen detection in water supplies is a significant challenge that must take into account: the representative volume of water that must be sampled and examined to have statistical confidence in the results; the methods used for concentrating the sample into reasonable volumes for analyses; the processing and purification steps needed for detection; the detection platform chosen (e.g. cell culture, PCR, fluorescent antibody); and, the engineering required to accomplish these tasks that will provide precise and accurate results with limited false positive and false negative error rates. The authors are currently testing a breadboard field device that was originally designed for aerosolized bioterrorism agents. However, the guiding biochemistry that drives the system can be readily adapted for water quality monitoring. The prototype field device is currently being developed for E. coli O157:H7. The device can autonomously sample up to 100 mL volumes of water. Processing to remove inhibitors is accomplished by flow-through immunomagnetic separation. The purified cells, immobilized on the magnetic beads, are then moved to a flow through PCR system, and PCR is performed directly off of the beads. The eluate is collected and the PCR sample is hybridized to an array with specific probes for the detection of these products. Both specificity and sensitivity of the automated protocol are excellent. Consistent recovery of 10 spiked cells into matrix water (Columbia River water) is routinely achieved. The system architecture and PCR thermal cycling protocols virtually eliminate PCR carryover allowing the system to be used many times before tubing and other easy user replaceable parts is required. The next generation system will be able to collect much larger volumes of water (up to 10 L), and link highly multiplexed PCR for multiple agents, with a flow through bead-array system for true autonomous detection of any waterborne pathogen. Includes 31 references, figures.