1.1本规程描述了在185至820 nm波长范围内估算分光光度计光谱带宽的程序。 1.2这些实践适用于所有利用计算机控制和数据处理的现代分光光度计设计。这包括常规光学设计，其中样品由单色光照射，以及耦合到光电二极管阵列的“反向”光学设计，其中光通过样品后由多色器分离。对于使用伺服的分光光度计- 操作狭缝，并在波长自动扫描时保持恒定的周期和恒定的信噪比，和/或利用固定狭缝，通过自动改变增益或动态节点电压来保持恒定的伺服回路增益，请参阅中所述的步骤 附件A1 . 该程序与本规程早期版本中描述的程序相同。 1.3本规程不包括极限光谱带宽的测量，其定义为在最佳实验条件下可实现的最小光谱带宽。 1.4以国际单位制表示的数值应视为标准值。本标准不包括其他计量单位。 1.5 本标准并非旨在解决与其使用相关的所有安全问题（如有）。本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。 ====意义和用途====== 4.1开发分析方法的人员应使用这些规程，以确保规程中引用的光谱带宽实际上是使用的带宽。 注释2 — 方法开发者应确定可用于获得满意结果的光谱带宽。 4.2应使用这些实践来确定是否可以使用给定的分光光度计实现方法中规定的光谱带宽，从而确定仪器是否适合在此应用中使用。如果要对具有尖锐吸收带（自然半带宽小于15 nm）的化合物进行准确的吸光度测量，所用光谱仪的光谱带宽应优于 1. / 第八 化合物吸收的自然半带宽。 4.3这些实践允许分光光度计的用户在给定的一组条件下估计仪器的实际光谱带宽，并将结果与根据制造商文献中给出的数据或仪器指示的数据计算的光谱带宽进行比较。

1.1 This practice describes procedures for estimating the spectral bandwidth of a spectrophotometer in the wavelength region of 185 to 820 nm. 1.2 These practices are applicable to all modern spectrophotometer designs utilizing computer control and data handling. This includes conventional optical designs, where the sample is irradiated by monochromatic light, and ‘reverse’ optic designs coupled to photodiode arrays, where the light is separated by a polychromator after passing through the sample. For spectrophotometers that utilize servo-operated slits and maintain a constant period and a constant signal-to-noise ratio as the wavelength is automatically scanned, and/or utilize fixed slits and maintain a constant servo loop gain by automatically varying gain or dynode voltage, refer to the procedure described in Annex A1 . This procedure is identical to that described in earlier versions of this practice. 1.3 This practice does not cover the measurement of limiting spectral bandwidth, defined as the minimum spectral bandwidth achievable under optimum experimental conditions. 1.4 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. ====== Significance And Use ====== 4.1 These practices should be used by a person who develops an analytical method to ensure that the spectral bandwidths cited in the practice are actually the ones used. Note 2 — The method developer should establish the spectral bandwidths that can be used to obtain satisfactory results. 4.2 These practices should be used to determine whether a spectral bandwidth specified in a method can be realized with a given spectrophotometer and thus whether the instrument is suitable for use in this application. If accurate absorbance measurements are to be made on compounds with sharp absorption bands (natural half band widths of less than 15 nm) the spectral bandwidth of the spectrometer used should be better than 1 / 8th of the natural half band width of the compound’s absorption. 4.3 These practices allow the user of a spectrophotometer to estimate the actual spectral bandwidth of the instrument under a given set of conditions and to compare the result to the spectral bandwidth calculated from data given in the manufacturer's literature or indicated by the instrument.