The way in which particulates, including organic matter, minerals and microorganisms, react with their environment is particularly important in the drinking water field. Understanding this environmental relationship starts with understanding the characteristics of the particle outermost surface fringes. Of particular importance is the particle's electrostatic charge which is directly relevant to the adsorption of ions, attraction to or repulsion from surfaces, suspension stability, adhesion, aggregation and flocculation. This paper summarizes the fundamental theories used to describe the surface charge of particles. Factors influencing the surface charge of particles in drinking water are described and relationships between them are illustrated using experimental results of studies employing the use of microorganisms. A discussion of precautions that must be taken during sample preparation and during surface charge measurements is included. Finally, a discussion of how surface charge measurements can be used as a water treatment process control tool is presented.