Conventional validation testing of ultraviolet (UV) reactors use cultured microorganisms
spiked into test water flowing through a reactor. The objective of this study was to examine
the UV inactivation of indigenous aerobic spores naturally occurring in raw/unfiltered
water supplies and to assess their use as an alternative indicator for validation testing of
UV reactor performance. Spores were found in all raw waters tested in concentrations
ranging between 20-12,000 CFU/100 ml and were very resistant to UV irradiation. The
inactivation of indigenous natural aerobic spores followed first order kinetics with an
inactivation coefficient ranging between 0.013-0.022 cm2/mJ. It was determined that
naturally occurring aerobic spores can be a useful tool when validating plant
performance, and can be used as a regular monitor of UV fluence and performance in a
water treatment plant. Includes table, figures.