Cosmetics - Microbiology - Detection of Pseudomonas aeruginosa (ISO 22717:2015)
化妆品 - 微生物学 - 铜绿假单胞菌检测(ISO 22717:2015)
发布日期:
2015-12-16
实施日期:
2015-12-16
ISO 22717:2015给出了化妆品中指定微生物铜绿假单胞菌的检测和鉴定的一般准则。按照国际惯例或法规,本国际标准规定的微生物可能因国家而异。
为了确保消费者的产品质量和安全,建议进行适当的微生物风险分析,以确定适用本国际标准的化妆品的类型。被认为具有低微生物(见ISO 29621)风险的产品包括水分低,含水酒精产品,极端pH值等。
本国际标准中描述的方法是基于在非选择性液体培养基(富集肉汤)中检测铜绿假单胞菌,然后在选择性琼脂培养基上分离。其他方法可能是适当的,这取决于所需的检测水平。
注意对于铜绿假单胞菌的检测,可以在非选择性培养基上进行亚文化培养,然后进行合适的鉴定步骤(例如使用鉴定试剂盒)。
由于在这一领域的各种化妆品的应用,这种方法可能不合适
ISO 22717:2015 gives general guidelines for the detection and identification of the specified microorganism Pseudomonas aeruginosa in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations.
In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic product to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc.
The method described in this International Standard is based on the detection of Pseudomonas aeruginosa in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate, depending on the level of detection required.
NOTE For the detection of Pseudomonas aeruginosa, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits).
Because of the large variety of cosmetic products within this field of application, this method may not be appropriate in every detail for some products (e.g. certain water immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.