ISO 21150:2015给出化妆品中特定微生物大肠杆菌的检测和鉴定的一般准则。按照国际惯例或法规，本国际标准规定的微生物可能因国家而异。 为了确保消费者的产品质量和安全，建议进行适当的微生物风险分析，以确定本国际标准适用的化妆品类型。被认为具有低微生物（见ISO 29621）风险的产品包括水分低，含水酒精产品，极端pH值等。 本国际标准中描述的方法基于在非选择性液体培养基（富集肉汤）中检测大肠杆菌，然后在选择性琼脂培养基上分离。其他方法可能是适当的，这取决于所需的检测水平。 注意对于大肠杆菌的检测，可以在非选择性培养基上进行传代培养，然后进行合适的鉴定步骤（例如使用鉴定试剂盒）。 由于这个应用范围内的化妆品种类繁多，这种方法可能不适合于某些产品

ISO 21150:2015 gives general guidelines for the detection and identification of the specified microorganism Escherichia coli in cosmetic products. Microorganisms considered as specified in this International Standard might differ from country to country according to national practices or regulations. In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis, so as to determine the types of cosmetic products to which this International Standard is applicable. Products considered to present a low microbiological (see ISO 29621) risk include those with low water activity, hydro-alcoholic products, extreme pH values, etc. The method described in this International Standard is based on the detection of Escherichia coli in a non-selective liquid medium (enrichment broth), followed by isolation on a selective agar medium. Other methods may be appropriate, depending on the level of detection required. NOTE For the detection of Escherichia coli, subcultures can be performed on non-selective culture media followed by suitable identification steps (e.g. using identification kits). Because of the large variety of cosmetic products within this field of application, this method might not be suited to some products in every detail (e.g. certain water-immiscible products). Other International Standards (ISO 18415) may be appropriate. Other methods (e.g. automated) can be substituted for the test presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.