CEN技术报告的这一部分通过培养大肠杆菌的生殖琼脂培养基上的个体菌落来定义膜过滤程序，用于定量检测。在污泥，土壤，土壤改良剂，成长中的媒体和生物群落。技术报告的这一部分不适用于其处理将显着地将细菌水平降低到每g湿重小于10个可行的大肠杆菌，例如石灰添加，干燥或巴氏消毒的材料。液体富集和最可能的数量估计方法可能适合于此目的。 这种膜过滤方法不适用于其他大肠菌群的计数和检测，而不改变生色琼脂培养基。 适用于通过处理评估大肠杆菌的对数减少以及最终产品的质量。 该方法适用于残留物残留少于20％的物质。干性残留物大于20％，大肠杆菌数量较少的材料应使用CEN/TR 15214-2和CEN/TR 15214-3

This part of the CEN Technical Report specifies a membrane filtration procedure for the quantitative detection, by culture of individual colonies on chromogenic agar media, of Escherichia coli. in sludges, soils, soil improvers, growing media and biowastes. This part of the Technical Report is not suitable for materials whose treatment will significantly reduce bacterial levels to less than 10 viable E. coli per g wet weight, such as lime addition, drying or pasteurisation. A liquid enrichment and most probable number estimation method may be suited for such purpose. This membrane filtration method is not appropriate for enumeration and detection of other coliform bacteria without modifications to the chromogenic agar media. It is suitable to evaluate the log reduction of E.coli through treatment, as well as the quality of the end product. This method is for materials with dry residues less than 20 %. For materials with dry residues greater than 20 % and low numbers of E. coli, CEN/TR 15214-2 and CEN/TR 15214-3 should be used.