范围:本文件提供了如何通过合作研究评估和验证食品中特定DNA序列检测的定性（二元）实时聚合酶链反应（PCR）方法的性能特征的信息。 本指南适用于用于检测转基因食品DNA序列的定性PCR方法的验证。它们也适用于用于检测食品中其他目标序列的PCR方法，例如用于物种检测和鉴定。 交叉引用:ISO 24276EN ISO 24276ISO 16577ISO 5725-2:1994EN ISO 21571:2005ISO 21571:2005ISO指南35:2017购买本文件时提供的所有当前修订版均包含在购买本文件中。

Scope: This document provides information on how the performance characteristics of qualitative (binary) real-time polymerase chain reaction (PCR) methods for detection of specific DNA sequences present in foods should be evaluated and validated by conducting a collaborative study. The guidelines are applicable for validation of qualitative PCR methods used for detection of DNA sequences derived from genetically modified foodstuffs. They can be applicable also for PCR methods used for detection of other target sequences in foodstuffs, e.g. for species detection and identification.Cross References:ISO 24276EN ISO 24276ISO 16577ISO 5725-2:1994EN ISO 21571:2005ISO 21571:2005ISO Guide 35:2017All current amendments available at time of purchase are included with the purchase of this document.