本文件描述了用于检测食品中特定DNA序列的定性（二元）实时聚合酶链反应（PCR）方法进行单一实验室验证研究的性能特征和最低性能标准。 该方案是为定性实时PCR方法开发的，用于检测来自转基因食品的DNA序列。它也适用于用于分析其他食品材料的定性PCR方法的单实验室验证，例如用于物种检测和鉴定。购买本文件时可获得的所有当前修订均包含在购买本文件中。

This document describes the performance characteristics and minimum performance criteria for conducting a single-laboratory validation study for qualitative (binary) real-time polymerase chain reaction (PCR) methods applied for the detection of specific DNA sequences present in foods. The protocol was developed for qualitative real-time PCR methods for the detection of DNA sequences derived from genetically modified foodstuffs. It is applicable also for single-laboratory validation of qualitative PCR methods used for analysis of other food materials, e.g. for species detection and identification.All current amendments available at time of purchase are included with the purchase of this document.