ISO 16256:2012描述了一种测试酵母菌抗真菌剂易感性的方法，包括念珠菌属。和新生隐球菌，导致感染。这里描述的参考方法尚未用于二倍体真菌的酵母形式的研究，例如双歧杆菌（Dermatitidis）和/或荚膜梭菌（H. capsulatum）荚膜菌。 ISO 16256:2012描述了可以通过两种途径之一实现的肉汤微量稀释参考方法。一个途径涉及MIC的视觉测定（CLSI方法）;第二个途径涉及分光光度法测定MIC（EUCAST方法）

ISO 16256:2012 describes a method for testing the susceptibility to antifungal agents of yeasts, including Candida spp. and Cryptococcus neoformans, that cause infections. The reference method described here has not been used in studies of the yeast forms of dimorphic fungi, such as B. dermatitidis and/or H. capsulatum variety capsulatum. ISO 16256:2012 describes the broth microdilution reference method which can be implemented by either of two pathways. One pathway involves visual determination of MICs (CLSI method); the second pathway involves spectrophotometric determination of MICs (EUCAST method).