本文件规定了使用反相高效液相色谱-串联质谱法（LC-MS/MS）在ISO 20778规定的强烈吸烟条件下，对香烟主流烟雾总颗粒物中四种烟草特有亚硝胺（TSNAs）进行定量的方法。定量的TSNA包括:N-亚硝基鸟嘌呤（NNN）、N-亚硝基鸟嘌呤（NAT）、N-亚硝基鸟嘌呤（NAB）和4-（甲基亚硝胺）-1-（3-吡啶基）-1-丁酮（NNK）。

This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of cigarette mainstream smoke with the intense smoking regime specified in ISO 20778 by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).