快速方便地检测水样中的大肠菌群和大肠杆菌，尤其是 在饮用水中，由显色和荧光底物产生的物质越来越多 全球范围内的接受度更高。为了检测大肠杆菌，荧光底物 马克杯包含在各种广泛使用的、随时可用的培养基中，例如 ReadyCurt®、Colilert®等。马克杯被Y-葡萄糖醛酸酶切割 存在于98%的大肠杆菌菌株中。然而，这方面存在着巨大的差距 大肠杆菌种类的检测。正是大肠杆菌O157:H7，最危险的 血清型可导致极低数量的人类严重感染，但不能 用这种方法可以在水样中检测到。这种血清型通常不会 产生Y-葡萄糖醛酸酶，这反过来导致缺乏荧光 在浓缩培养液中的发展。为了填补这一空白，应用 简单的生化吲哚试验和随后的Singlepath®E.coli O157检测 新开发的具有改进特异性的侧向流动试验采用加标法进行试验 用ReadyClult®浓缩18-24小时后的水样。 自来水样本 在不同浓度（2-120 CFU/100 ml）下加入43种不同的细菌 不同的大肠杆菌和非大肠杆菌分离物。培养后，染色和荧光 对发展过程进行了可视化分析。正如预期的那样，所有10种被检测的大肠杆菌O157:H7都导致 颜色显影表明它们属于大肠菌群，但呈荧光 阴性，表明它们不代表大肠杆菌。然而，他们都给出了答案 应用吲哚试验的阳性结果表明它们属于大肠杆菌。这个 直接使用小容量（150微升； 没有煮沸）的文化 Singlepath®E.coli O157专门检测了所有含有E.coli O157的样本 尖刺。只有这些大肠杆菌O157菌株在5分钟内产生了明显的阳性结果 总之，水中存在高致病性大肠杆菌O157:H7 在即用培养基中浓缩后的样品可以通过 使用廉价的吲哚测试。如果呈阳性，则可以检测到Singlepath®大肠杆菌O157 直接使用，无需进一步制备样品。 包括桌子。

Rapid and convenient detection of Coliforms and E. coli in water samples, especially in drinking water, by chromogenic and fluorogenic substrates is gaining more and more acceptance worldwide. For the detection of E. coli, the fluorogenic substrate MUG is included in various widely used, ready-to-use culture media such as ReadyCult®, Colilert®, and others. MUG is cleaved by Y-glucuronidase which is present in 98% of all E. coli strains. However, there is a significant gap in the detection of the variety of E. coli. Exactly E. coli O157:H7, the most dangerous serotype, which can lead in very low numbers to serious infections in humans, cannot be detected in water samples by this method. This serotype typically does not produce Y-glucuronidase and this in turn results in a lack of fluorescence development in the enriched culture broth. In order to fill this gap, the application of the simple biochemical indole test and subsequently of Singlepath® E. coli O157, a newly developed lateral flow test with improved specificity, was tested with spiked water samples after enrichment with ReadyCult® for 18-24 h. Tap water samples were spiked at various levels (2-120 CFU/100 ml) with 43 different bacteria belonging to various E. coli and non-E. coli isolates. After culturing, color and fluorescence development was visually analyzed. As expected, all 10 E. coli O157:H7 tested led to color development indicating their belonging to the Coliforms, but were fluorescence negative, indicating that they do not represent E. coli. However, all of them gave positive results with the indole test applied indicating their belonging to E. coli. The direct application of a small volume (150 ul; without boiling) of the culture to the Singlepath® E. coli O157 specifically detected all samples in which E. coli O157 were spiked. Only these E. coli O157 strains led to a clear positive result mostly within 5 min. In summary, the presence of highly pathogenic E. coli O157:H7 in water samples after enrichment in ready-to-use culture media can be easily detected by using an inexpensive indole test. If positive, Singlepath® E. coli O157 can then be applied directly without further preparation of the sample. Includes table.