1.1这些实践包括叶绿素的提取和测量 a、 b、， 和 c 和脱镁素 一 在淡水和海洋浮游生物和附生植物中。提供了以下三种做法: 1.1.1测量叶绿素的分光光度法、三色实施规程 a、 b、， 和 c . 1.1.2测量叶绿素的分光光度法、单色实施规程 一 修正为脱镁素 一 ; 用于测量脱镁素 一 . 1.1.3测量叶绿素的荧光测量规程 一 修正为脱镁素 一 ; 用于测量脱镁素 一 . 1.2以国际单位制表示的数值应视为标准。括号中给出的值仅供参考。 1.3 本标准并非旨在解决与其使用相关的所有安全问题（如有）。 本标准的用户有责任在使用前制定适当的安全和健康实践，并确定监管限制的适用性。 第节给出了具体的预防说明 7. . ====意义和用途====== 4.1藻类叶绿素含量数据具有以下应用: 4.1.1提供藻类生物量和生产力的估计。 4.1.2根据叶绿素的相对含量，提供藻类分类组成（主要类群）的一般信息 a、 b、， 和 c 和藻类群落的生理条件，这与藻段的相对丰度有关。 4.1.3确定水质的长期趋势。 4.1.4确定地表水的营养状态。 4.1.5检测污染物对受纳水体中浮游生物和附生植物的不利影响。 4.1.6确定藻类生长潜力试验中的最大生长速率和产量。

1.1 These practices include the extraction and the measurement of chlorophyll a, b, and c , and pheophytin a in freshwater and marine plankton and periphyton. Three practices are provided as follows: 1.1.1 Spectrophotometric, trichromatic practice for measuring chlorophyll a, b, and c . 1.1.2 Spectrophotometric, monochromatic practice for measuring chlorophyll a corrected for pheophytin a ; and for measuring pheophytin a . 1.1.3 Fluorometric practice for measuring chlorophyll a corrected for pheophytin a ; and for measuring pheophytin a . 1.2 The values stated in SI units are to be regarded as the standard. The values given in parentheses are provided for information purposes only. 1.3 This standard does not purport to address all of the safety problems, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use. Specific precautionary statements are given in Section 7 . ====== Significance And Use ====== 4.1 Data on the chlorophyll content of the algae have the following applications: 4.1.1 To provide estimates of algal biomass and productivity. 4.1.2 To provide general information on the taxonomic composition (major groups) of the algae, based on the relative amounts of chlorophyll a, b, and c , and the physiological condition of algal communities, which is related to the relative abundance of pheopigments. 4.1.3 To determine long-term trends in water quality. 4.1.4 To determine the trophic status of surface waters. 4.1.5 To detect adverse effects of pollutants on plankton and periphyton in receiving waters. 4.1.6 To determine maximum growth rates and yields in algal growth potential tests.