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现行 CEN ISO/TS 6579-2:2012
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Microbiology of food and animal feed - Horizontal method for the detection, enumeration and serotyping of Salmonella - Part 2: Enumeration by a miniaturized most probable number technique (ISO/TS 6579-2:2012) 食品和动物饲料的微生物学 - 沙门氏菌检测 计数和血清分型的水平方法 - 第2部分:通过小型化最可能的数字技术(ISO/TS 6579-2:2012)
发布日期: 2012-11-01
实施日期: 2012-11-01
ISO/TS 6579-2给出了计数沙门氏菌的方法。存在于:用于人类消费和饲养动物的产品;食品生产和食品处理领域的环境样品;动物粪便以及通过计算最可能数(MPN)的初级生产阶段的环境样品。 该方法基于稀释,预富集和选择性富集步骤的小型化。选择性富集培养基,改性半固体Rappaport?Vassiliadis(MSRV),用于检测运动性沙门氏菌,不适合检测非运动性沙门氏菌。 该方法可能不太适合枚举沙门氏菌。伤寒沙门氏菌副伤寒 该方法不适用于计数沙门氏菌。 (非常)低污染的样品(<1 cfu/g)
ISO/TS 6579-2 gives a method for the enumeration of Salmonella spp. present in: products intended for human consumption and for the feeding of animals; environmental samples in the area of food production and food handling; animal faeces; and environmental samples from the primary production stage by calculation of the most probable number (MPN). The method is based on miniaturization of the dilution, pre-enrichment and selective enrichment steps. The selective enrichment medium, modified semi-solid Rappaport?Vassiliadis (MSRV), is intended for the detection of motile salmonellae and is not appropriate for the detection of non-motile salmonellae. It is possible that the method is less appropriate to enumerate Salmonella ser. Typhi and Salmonella ser. Paratyphi. The method is not appropriate for the enumeration of Salmonella spp. in (very) low contaminated samples (<1 cfu/g).
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归口单位: CEN/TC 275-
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