本文件规定了一种通过测量释放的二氧化碳的量来确定塑料材料(包括含有制剂添加剂的塑料材料)的有氧生物降解性程度的方法。测试材料在标准化实验室条件下在合成培养基中暴露于好氧条件下来自活性污泥的接种物。 本文件中使用的条件不一定对应于允许发生最大生物降解的最佳条件，但该测试方法旨在测量塑料材料的生物降解并给出其潜在生物降解性的指示。 该方法能够通过计算碳平衡（可选，见附件？C）来改进对生物降解的评估。 该方法适用于以下材料:—？天然和/或合成聚合物、共聚物或其混合物； —？含有添加剂如增塑剂、着色剂或其它化合物的塑料材料； —？水溶性聚合物； —？在试验条件下不抑制接种物中存在的微生物的材料。可以使用抑制对照或通过另一种合适的方法(参见例如ISO 8192[1])测定抑制作用。如果测试材料对接种物有抑制作用，则可以使用较低的测试浓度、另一种接种物或预先暴露的接种物。

This document specifies a method, by measuring the amount of carbon dioxide evolved, for the determination of the degree of aerobic biodegradability of plastic materials, including those containing formulation additives. The test material is exposed in a synthetic medium under standardized laboratory conditions to an inoculum from activated sludge under aerobic conditions. The conditions used in this document do not necessarily correspond to the optimum conditions allowing maximum biodegradation to occur, but this test method is designed to measure the biodegradation of plastic materials and give an indication of their potential biodegradability. The method enables the assessment of the biodegradation to be improved by calculating a carbon balance (optional, see Annex？C). The method applies to the following materials: —？natural and/or synthetic polymers, copolymers or mixtures thereof; —？plastic materials which contain additives such as plasticizers, colorants or other compounds; —？water-soluble polymers; —？materials which, under the test conditions, do not inhibit the microorganisms present in the inoculum. Inhibitory effects can be determined using an inhibition control or by another appropriate method (see, for example, ISO？8192^[1]). If the test material is inhibitory to the inoculum, a lower test concentration, another inoculum or a pre-exposed inoculum can be used.