臭氧和二氧化氯等消毒剂已被证明对微小隐孢子虫的灭活有效，而游离氯和一氯胺等消毒剂在单独使用时需要过多的接触时间才能达到相当的灭活水平。在过去几年中，人们探索了在使用臭氧或二氧化氯等更强的化学消毒剂后，连续使用氯物种（例如游离氯、一氯胺），作为控制微小隐孢子虫的替代方法。一般来说，二级消毒剂性能的提高被认为是某些消毒方案中该过程最重要的影响。 本研究的目的是研究在低温（1-20摄氏度）下，臭氧和游离氯相继灭活微小隐球菌的协同作用。使用改良的体外脱囊法评估经处理的卵囊的生存能力，该方法已被证明与先前研究中的动物传染性数据一致。包括14个参考文献、图表。

Disinfectants like ozone and chlorine dioxide have been shown to beeffective in inactivating Cryptosporidium parvum, whereas others like free chlorine and monochloramine, require excessively high contact times to achieve comparable levels of inactivation when applied alone. Sequential application of chlorine species (e.g. free chlorine, monochloramine) after a stronger chemical disinfectant like ozone or chlorine dioxide, has been explored in the last few years as an alternative to control C. parvum. In general, an enhancement in the performance of the secondary disinfectant has been found to be the most important effect of this process for some disinfection schemes. The objective of this study was to investigate the synergy involved in the sequential inactivation of C. parvum with ozone followed by free chlorine at low temperature (1-20 degrees C). Viability of treated oocysts was assessed using the modified in vitro excystation method that has been shown to be consistent with animal infectivity data in previous studies. Includes 14 references, figures.