BS ISO 21115:2019规定了使用虹鳟（虹鳟）鳃的永久细胞系RTgill-W1测定鱼类急性毒性的方法。将24孔组织培养板中汇合单层中的细胞暴露于水样（如地表水或不同类型的废水）或化学品中24小时，然后根据荧光细胞活力指示染料评估细胞活力（见4.1）。然后，将数据表示为未暴露对照和毒性的百分比，根据响应曲线中细胞存活率与出水百分比或化学浓度的百分比进行量化（见第9条）。 交叉引用:ISO 15088OECD 236购买时可用的所有当前修订均包含在本文件的购买中。

BS ISO 21115:2019 specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).Cross References:ISO 15088OECD 236All current amendments available at time of purchase are included with the purchase of this document.