本文件规定了使用虹鳟鱼鳃永久性细胞系RTgill-W1测定鱼类急性毒性的方法。将24孔组织培养板中融合单层中的细胞暴露于水样（如地表水或不同种类的废水）或化学品中24小时，然后根据荧光细胞活力指示染料评估细胞活力（见4.1）。然后，将数据表示为未暴露对照组的百分比，并根据细胞存活率与反应曲线中的出水百分比或化学品浓度进行量化（见第9条）。

This document specifies a method for the determination of fish acute toxicity using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. Cells in confluent monolayers in 24-well tissue culture plates are exposed to water samples, such as surface waters or different kinds of effluents, or to chemicals for 24 h and, thereafter, cell viability is assessed based on fluorescent cell viability indicator dyes (see 4.1). Data are then expressed as a percentage of unexposed control and toxicity quantified based on the percentage of cell viability versus the percentage of effluent or the chemical concentration in response curves (see Clause 9).